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eagle eye ii system  (Agilent technologies)


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  • 90

    Structured Review

    Agilent technologies eagle eye ii system
    Eagle Eye Ii System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eagle+eye+ii/pm39057432-430-29-33
    Average 90 stars, based on 1 article reviews
    eagle eye ii system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Polyacrylamide Gel Electrophoresis:

    Article Title: Evolving new molecular function
    Article Snippet: DNA oligonucleotides were synthesized on a PerSeptive Biosystems Expedite 8909 DNA synthesizer using standard protocols and purified by reverse phase HPLC. .. Oligonucleotides were quantitated spectrophotometrically and by denaturing polyacrylamide gel electrophoresis (PAGE) followed by staining with ethidium bromide or SYBR Green (Molecular Probes) and quantitation using a Stratagene Eagle Eye II densitometer. .. Phosphoramidites enabling the synthesis of 5′-NH2-dT, 5′ tetrachlorofluorescein, abasic backbone spacer, C3 backbone spacer, 9-bond polyethylene glycol spacer, 12-bond saturated hydrocarbon spacer, and 5′ biotin groups were purchased from Glen Research, Sterling, Va., USA.

    Staining:

    Article Title: Evolving new molecular function
    Article Snippet: DNA oligonucleotides were synthesized on a PerSeptive Biosystems Expedite 8909 DNA synthesizer using standard protocols and purified by reverse phase HPLC. .. Oligonucleotides were quantitated spectrophotometrically and by denaturing polyacrylamide gel electrophoresis (PAGE) followed by staining with ethidium bromide or SYBR Green (Molecular Probes) and quantitation using a Stratagene Eagle Eye II densitometer. .. Phosphoramidites enabling the synthesis of 5′-NH2-dT, 5′ tetrachlorofluorescein, abasic backbone spacer, C3 backbone spacer, 9-bond polyethylene glycol spacer, 12-bond saturated hydrocarbon spacer, and 5′ biotin groups were purchased from Glen Research, Sterling, Va., USA.

    Article Title: Olfactomedin 4 promotes S‐phase transition in proliferation of pancreatic cancer cells
    Article Snippet: The stained cells were photographed with a BX51 microscope (Olympus, Tokyo, Japan) and the image was acquired with a DP‐70 digital camera (Olympus) and software DP controller version 1.2 (Olympus). .. The whole area of stained cells in the chamber slide was also photographed with Eagle Eye II (Stratagene, LaJolla, CA, USA) with trans‐fluorescence light. ..

    SYBR Green Assay:

    Article Title: Evolving new molecular function
    Article Snippet: DNA oligonucleotides were synthesized on a PerSeptive Biosystems Expedite 8909 DNA synthesizer using standard protocols and purified by reverse phase HPLC. .. Oligonucleotides were quantitated spectrophotometrically and by denaturing polyacrylamide gel electrophoresis (PAGE) followed by staining with ethidium bromide or SYBR Green (Molecular Probes) and quantitation using a Stratagene Eagle Eye II densitometer. .. Phosphoramidites enabling the synthesis of 5′-NH2-dT, 5′ tetrachlorofluorescein, abasic backbone spacer, C3 backbone spacer, 9-bond polyethylene glycol spacer, 12-bond saturated hydrocarbon spacer, and 5′ biotin groups were purchased from Glen Research, Sterling, Va., USA.

    Quantitation Assay:

    Article Title: Evolving new molecular function
    Article Snippet: DNA oligonucleotides were synthesized on a PerSeptive Biosystems Expedite 8909 DNA synthesizer using standard protocols and purified by reverse phase HPLC. .. Oligonucleotides were quantitated spectrophotometrically and by denaturing polyacrylamide gel electrophoresis (PAGE) followed by staining with ethidium bromide or SYBR Green (Molecular Probes) and quantitation using a Stratagene Eagle Eye II densitometer. .. Phosphoramidites enabling the synthesis of 5′-NH2-dT, 5′ tetrachlorofluorescein, abasic backbone spacer, C3 backbone spacer, 9-bond polyethylene glycol spacer, 12-bond saturated hydrocarbon spacer, and 5′ biotin groups were purchased from Glen Research, Sterling, Va., USA.

    other:

    Article Title: Assessment of human adenovirus removal in a full-scale membrane bioreactor treating municipal wastewater.
    Article Snippet: Human adenoviruses (HAdVs) in wastewater samples taken from four different treatment stages of a full-scale municipal wastewater treatment plant (i.e., incoming raw sewage, primary sedimentation effluent, membrane bioreactor (MBR) influent, and MBR effluent) were quantified by real-time PCR assays to further estimate removal efficiency of the HAdVs.. Based on hexon gene sequence comparisons, HAdV species A, C, and F were consistently found in the wastewater samples.. In general, all three identified HAdV species were detected in most of the wastewater samples using the real-time PCR assays.

    Article Title: Cell attachment and proliferation of bone marrow-derived osteoblast on zirconia of various surface treatment
    Article Snippet: The intensity of the bands was quantified under UV transillumination (Eagle Eye II, Stratagene, La Jolla, CA, USA).

    Article Title: Genome organization and assessment of high copy number and increased expression of pectinolytic genes from Penicillium griseoroseum: a potential heterologous system for protein production.
    Article Snippet: Protoplast preparation and analysis by pulsed-field gel electrophoresis (PfGe) Protoplasts were obtained according to the method proposed by Dias et al. [12] with modifications. a 5 ml volume of osmotic stabilizer (10 mM phosphate buffer, 0.6 M Kcl) and 30 mg of lysing enzymes l1412 (sIGMa®) was added to 800 mg of mycelium and incubated at 80 rpm and 30 °c for a period of 3–4 h. the protoplasts were then filtered, washed with osmotic stabilizer, and centrifuged at 2300g for 5 min. a suspension of 108 protoplasts/ml was mixed with an equal volume of 1.4 % agarose solution at 50 °c. the blocks of agarose containing the protoplasts were transferred to microtubes containing 1 ml of nDs solution (1 % N-lauroyl sarcosine, 50 mM eDta, pH 8.0, 10 mM tris–Hcl) and 50 μl proteinase K (20 mg/ml) and incubated at 50 °c for 48 h. the blocks were washed twice in 50 mM eDta (pH 8.0) at 50 °c for 30 min, and the tubes containing the blocks were stored at 4 °c until use. the gel was prepared with 0.8 % chromosomal-grade agarose (Bio-rad) in 0.5× tBe (45.0 mM tris–borate, 1.0 mM eDta, pH 8.0). the buffer was changed every 48 h and maintained at constant temperature (10 °c). the separation was performed in a cHef-DrII system from Bio-rad with a current of 1.2 V/cm and pulses divided into three blocks: B1 8400–13000 s, 75 h; B2 3300–5400 s, 90 h; B3 2000–2700, 45 h. after chromosome separation, the gel was treated with 100 ml of distilled water containing 5 μl of ethidium bromide solution (10 mg/ml) for 1 3 30–60 min and then placed in distilled water for at least 30 min. the gel was then analyzed, and the image was scanned with the eagle eye II (stratagene) photo-documentation device.



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