eagle eye ii system (Agilent technologies)
90
Structured Review
Agilent technologies
eagle eye ii system
Eagle Eye Ii System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eagle+eye+ii/pm39057432-430-29-33
Average 90 stars, based on 1 article reviews
Eagle Eye Ii System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eagle+eye+ii/pm39057432-430-29-33
Average 90 stars, based on 1 article reviews
eagle eye ii system - by Bioz Stars,
2026-09
90/100 stars
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Polyacrylamide Gel Electrophoresis:Article Title: Evolving new molecular function Article Snippet: DNA oligonucleotides were synthesized on a PerSeptive Biosystems Expedite 8909 DNA synthesizer using standard protocols and purified by reverse phase HPLC. .. Oligonucleotides were quantitated spectrophotometrically and by denaturing polyacrylamide gel electrophoresis (PAGE) followed by staining with ethidium bromide or SYBR Green (Molecular Probes) and quantitation using a Stratagene Staining:Article Title: Evolving new molecular function Article Snippet: DNA oligonucleotides were synthesized on a PerSeptive Biosystems Expedite 8909 DNA synthesizer using standard protocols and purified by reverse phase HPLC. .. Oligonucleotides were quantitated spectrophotometrically and by denaturing polyacrylamide gel electrophoresis (PAGE) followed by staining with ethidium bromide or SYBR Green (Molecular Probes) and quantitation using a Stratagene Article Title: Olfactomedin 4 promotes S‐phase transition in proliferation of pancreatic cancer cells Article Snippet: The stained cells were photographed with a BX51 microscope (Olympus, Tokyo, Japan) and the image was acquired with a DP‐70 digital camera (Olympus) and software DP controller version 1.2 (Olympus). .. The whole area of stained cells in the chamber slide was also photographed with SYBR Green Assay:Article Title: Evolving new molecular function Article Snippet: DNA oligonucleotides were synthesized on a PerSeptive Biosystems Expedite 8909 DNA synthesizer using standard protocols and purified by reverse phase HPLC. .. 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[12] with modifications. a 5 ml volume of osmotic stabilizer (10 mM phosphate buffer, 0.6 M Kcl) and 30 mg of lysing enzymes l1412 (sIGMa®) was added to 800 mg of mycelium and incubated at 80 rpm and 30 °c for a period of 3–4 h. the protoplasts were then filtered, washed with osmotic stabilizer, and centrifuged at 2300g for 5 min. a suspension of 108 protoplasts/ml was mixed with an equal volume of 1.4 % agarose solution at 50 °c. the blocks of agarose containing the protoplasts were transferred to microtubes containing 1 ml of nDs solution (1 % N-lauroyl sarcosine, 50 mM eDta, pH 8.0, 10 mM tris–Hcl) and 50 μl proteinase K (20 mg/ml) and incubated at 50 °c for 48 h. the blocks were washed twice in 50 mM eDta (pH 8.0) at 50 °c for 30 min, and the tubes containing the blocks were stored at 4 °c until use. the gel was prepared with 0.8 % chromosomal-grade agarose (Bio-rad) in 0.5× tBe (45.0 mM tris–borate, 1.0 mM eDta, pH 8.0). the buffer was changed every 48 h and maintained at constant temperature (10 °c). the separation was performed in a cHef-DrII system from Bio-rad with a current of 1.2 V/cm and pulses divided into three blocks: B1 8400–13000 s, 75 h; B2 3300–5400 s, 90 h; B3 2000–2700, 45 h. after chromosome separation, the gel was treated with 100 ml of distilled water containing 5 μl of ethidium bromide solution (10 mg/ml) for 1 3 30–60 min and then placed in distilled water for at least 30 min. the gel was then analyzed, and the image was scanned with the |